Abstract
In previous papers 1 , 2 it was shown that horse proteins injected intravenously into normal dogs are retained quantitatively in the canine circulation for at least 6 days. During this retention, however, the proteins undergo marked denaturization. By the end of 4 days they will call forth no recognizable anaphylactic reaction even on massive transfusion into hypersensitive dogs. By the end of 6 days they cease to act as sensitizing agents for normal dogs. We have endeavored to follow subsequent changes in these proteins.
Titration by means of a specific horse-protein-precipitating rabbit serum indicate that the proteins are further altered, denaturized or inhibited. By the end of 30 days they give little or no demonstrable reaction with rabbit precipitin, if tested in low dilutions.
That this negative reaction is not due to an absence of horse proteins from the 30-day blood sample is indicated by continuing the titration to higher dilutions (Table I). In dilutions above 1:1000, the 6-day and 30-day samples give practically identical precipitates and turbidities.
The horse proteins thus further altered are apparently retained quantitatively for at least 4 months (latest titration thus far made). Within the limits of the experimental error the 4-month and 30-day blood samples give identical precipitates in all dilutions.
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