Abstract
Living non-pathogenic bacteria appear in the thoracic duct lymph when these microorganisms are placed in the lumen of the duodenum in an alkalinized bile solution in the post-digestive dog. 1 , 2 Various concentrations of bile have been placed in the stomach of dogs through a fistulous opening and the gastric contents removed and titrated at intervals 2 to 3 hours after the bile was administered. Dehydrated bile (Difco) has been used, 100 cc. volume of fluid, concentration of bile varying from 0.5 to 10.0%. It was found that 5.0% and higher concentrations caused vomiting within a short period of time, followed by diarrhea. There was usually a period of active HCl secretion following the emptying of the stomach in this manner. One half of the required amount of acid was added to neutralize the alkali in 100 cc. of a 5.0% bile solution to see if this mixture would leave the stomach rapidly and not stimulate acid secretion. (Line “D” in graph.) One per cent bile was found to be the optimum concentration to rapidly leave the stomach and be followed by a certain period of acid deficit. (Line “B” in graph.) Three dogs were used for these experiments. Each curve on the graph is an average of 5 experiments using 3 dogs, a total of 15 separate experiments. Experiments were conducted during the forenoon. Animals were fed in the afternoon and used on alternating dogs 18 hours after feeding.
These animals had, in addition to the gastric fistula, a caecal fistula. 2 B. prodigiosus was suspended in water and the various bile solutions and the relative concentration in the caecum was determined. This method has been used to determine the relative bacterial killing power of the intestinal tract. 2
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