Abstract
The object of this paper is to study the restoration of the several fractions into which the plasma globulins and albumins may be divided, after severe bleeding of dogs. No general anesthetic was used in the experiments. The dogs were bled from a large vein either in the hind or front leg. Plasma-pharesis was employed to maintain the cell volume and blood viscosity at a high level.
The fractionating of the plasma proteins was done by various concentrations of sodium sulphate at a pH of 7. The nitrogen in each fraction was determined by the macro-Kjeldahl method and titration carried out with N/20 alkali.
The average results from the successful experiments on six dogs indicated that all the globulin fractions decrease more rapidly during and immediately after bleeding. After a lapse of 3 to 4 days, depending upon the severity of the bleeding, the globulins appear to be restored more rapidly. This shift in regeneration greatly influences the albumin-globulin ratio.
The total protein nitrogen was determined by the macro-Kjeldahl method; the non-protein nitrogen, by the technique of Folin. The fractionation of the proteins into fibrin, euglobulin, pseudoglobulin, and the three albumin fractions was carried out according to an unpublished method of Berglund. The nitrogen in each fraction was determined by a macro-Kjeldahl digestion and titration with N/20 alkali.
Table I is illustrative of the type of result obtained in the experiments.
It is to be clearly understood that the fractions of globulins and albumins shown in Table I are not to be considered as definite protein entities. Hersfeld and Klinger 1 , 2 , 3 believe that the protein fractions do not have a chemical individuality but are an interrelated series of colloidal particles of different degrees of dispersion, which can be transposed one into the other.
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