Abstract
A canula was placed in the thoracic duct of dogs under local anesthetic. Lymph was collected for 5 to 10 minutes. The duodenum was exposed through the anterior median line incision in the abdominal wall after local anesthetic had been applied. Forty cc. of solution containing suspended bacteria were injected with proper care and precautions into the lumen of the duodenum. Lymph was collected continuously and divided at 5 minute intervals in separate sterile tubes. The duodenum was immediately replaced and the small incision closed. The experiment was continued for 2 hours. Autopsy was performed to exclude injured duodenal mucosa. Phosphate buffered solutions (pH 8.0) and normal salt solutions were used. Fresh egg white, dog's serum and bile were added to these solutions to make a 10% concentration. B. prodigiosus and B. coli were suspended in these solutions. The table gives results of experiments with 40 dogs, approximately 5 dogs for each set of experiments.
The duodenum is permeable to living bacteria when the contents are suddenly alkalinized in the presence of a foreign protein; this also takes place when alkalinized bile is placed in the lumen of this part of the intestinal tract. Bile and normal salt solution with bacteria are associated with a slight degree of permeability. This is a preliminary report upon the problem of vaccination by oral ingestion of the antigen.
Get full access to this article
View all access options for this article.