Abstract
Rabbits were immunized against crystallized egg albumin. The antiserum produced was separated into the protein fractions, euglobulin, pseudoglobulin, and albumin by 33 per cent, 46 per cent, 64 per cent, and 100 per cent saturation with (NH4)2 SO4. A precipitin test by the contact method was made on the whole serum and on each protein fraction, in an effort to discover with which fraction the precipitin came down. In all 10 cases the whole serum gave a positive test, with precipitin titers ranging from 100,000 in 2 cases to 10,000,000 in 1 case. The precipitin was carried down with the euglobulin fraction, but during dialysis the pseudoglobulin was split off by hydrolysis and the precipitin remained in this water soluble fraction. The albumin fractions were found free of precipitins in all cases. The precipitin titer was less in the globulin fraction containing the precipitin than in the whole serum, calculated on the basis of equivalent globulin content.
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