cDNA Cloning and Sequence Analysis of Preproendothelin-1 (PPET-1) from Salmon,Oncorhynchus keta

Abstract

The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 (26%, 29%, 24%, and 39%, respectively).

Get full access to this article

View all access options for this article.

References

Rubanyi GM, Polokoff MA. Endothelins: molecular biology, biochemistry, pharmacology, physiology, and pathophysiology. Pharmacol Rev 46:325–415, 1994.

Cody WL, Doherty AM. The development of potent peptide agonists and antagonists for the endothelin receptors. Biopolymers 37:89–104, 1995.

Saida K, Mitsui Y, Ishida N. A novel peptide, vasoactive intestinal contractor, of a new (endothelin) peptide family: molecular cloning, expression, and biological activity. J Biol Chem 264:14,613–14,616, 1989.

Kurama M, Ishida N, Matsui M, Saida K, Mitsui Y. Sequence and neuronal expression of mouse endothelin-1 cDNA. Biochim Biophys Acta 1307:249–253, 1996.

Saida K, Hashimoto M, Mitsui Y, Ishida N, Uchide T. The prepro vasoactive intestinal contractor (VIC)/endothelin-2 gene (EDN2): structure, evolution, production, and embryonic expression. Genomics 64:51–61, 2000.

Uchide T, Uchide T, Adur J, Yoshioka K, Sasaki T, Temma K, Saida K. Endothelin-1 in smooth muscle cells and mast cells of mouse uterus after parturition. J Mol Endocrinol 27:165–73, 2001.

Kozakai T, Sakate M, Masuo Y, Uchide T, Saida K. Increased gene expression of endothelin-1 and vasoactive intestinal contractor/endothelin-2 in the mammary gland of lactating mice. Biochem Biophys Res Commun 297:1339–1343, 2002.

Masuo Y, Ishikawa Y, Kozakai T, Uchide T, Komatsu Y, Saida K. Vasoactive intestinal contractor/endothelin-2 gene expression in the murine central nervous system. Biochem Biophys Res Commun 300: 661–668, 2003.

Adur J, Takizawa S, Quan J, Uchide T, Saida K. Increased gene expression and production of murine endothelin receptors after birth. Biochem Biophys Res Commun 305:700–706, 2003.

10.

Quan J, Uchide T, Takizawa S, Adur J, Nara E, Saida K. cDNA cloning and sequence Analysis of Xenopus laevis preproendothelin-1. J Cardiovasc Pharmacol 1:S256–S259, 2004.

11.

Kotake-Nara E, Takizawa S, Quan J, Wang H, Saida K. Cobalt chloride induces neurite outgrowth in rat pheochromocytoma PC-12 cells through regulation of endothelin-2/vasoactive intestinal contractor. J Neurosci Res 81:563–571, 2005.

12.

Takizawa S, Uchide T, Adur J, Kozakai T, Kotake-Nara E, Quan J, Saida K. Differential expression of endothelin-2 along the mouse intestinal tract. J Mol Endocrinol 35:201–209, 2005.

13.

Uemura H, Naruse M, Naruse K, Hirohama T, Demura H, Kasuya Y. Immunoreactive endothelin in plasma of nonmammalian vertebrates. J Cardiovasc Pharmacol 17:S414–S416, 1991.

14.

Stenslokken KO, Sundin L, Nilsson GE. Endothelin receptors in teleost fishes: cardiovascular effects and branchial distribution. Am J Physiol Regul Integr Comp Physiol 290:R852–R856, 2006.

15.

Olson KR, Duff DW, Farrell AP, Keen J, Kellogg MD, Kullman D, Villa J. Cardiovascular effects of endothelin in trout. Am J Physiol 260(4 Pt 2):H1214–H1223, 1991.

16.

Poder TC, Silberberg SD, Rampe D. Contraction of reptile, amphibian, and fish blood vessels by endothelin-1. Can J Physiol Pharmacol 69: 215–217, 1991.

17.

Wang Y, Olson KR, Smith MP, Russell MJ, Conlon JM. Purification, structural characterization, and myotropic activity of endothelin from trout, Oncorhynchus mykiss. Am J Physiol 277(6 Pt 2):R1605–R1611, 1999.

18.

Miller CT, Schilling TF, Lee K, Parker J, Kimmel CB. Sucker encodes a zebrafish Endothelin-1 required for ventral pharyngeal arch development. Development 127:3815–3828, 2000.

19.

Nielsen H, Engelbrecht J, Brunak S, von Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10:1–6, 1997.

20.

Itoh Y, Yanagisawa M, Ohkubo S, Kimura C, Kosaka T, Inoue A, Ishida N, Mitsui Y, Onda H, Fujino M, Masaki T. Cloning and sequence analysis of cDNA encoding the precursor of a human endothelium-derived vasoconstrictor peptide, endothelin: identity of human and porcine endothelin. FEBS Lett 231:440–444, 1988.