Abstract
The need of large amounts of Pneumococci for certain immunological and chemical investigations led to the devising of the following methods:
The problem divided itself into three phases: 1. The discovery of a simple medium, easy to prepare and relatively inexpensive, which would allow of a vigorous growth of the organisms. 2. The devising of an apparatus permitting growth in bulk. 3. The recovery of the organisms from the culture medium.
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