Abstract
Following the method of perfusion introduced by Clark 1 , the effect of pancreatic perfusates upon the carbohydrate metabolism of entire animals was studied. The respiratory metabolism, the blood sugar, and the D:N ratio were studied in cats and dogs after depancreatization.
Perfusates were made by perfusing aseptically and at body temperature the pancreases of cats, dogs, pigs, and oxen with Locke's solution. The first perfusates were made with an alkaline medium, later perfusates were made with Locke's solution modified by the substitution of HCl varying in strength from 0.1 per cent. to 0.7 per cent. for sodium bicarbonate.
Respiratory quotients were obtained by a modified Jaquet method, the animal being confined in a respiration chamber through which a continuous stream of air was drawn for ventilation and from which a continuous sample of air was removed for analysis. In a later modification the stream of air from the respiration chamber was directed into a large gasometer for a twenty-minute period and the samples for analysis withdrawn from this total volume. Analyses were made by the Henderson and Bailey modification of Haldane's apparatus. The respiration chamber was frequently checked by analyses of outside air and by burning alcohol in it.
D :N ratios and respiratory quotients typical for cliahetes were obtained in the depancreatized animals.
Following the administration of sugar in the form of destrose or sucrose by stomacll tube pancreatic perlusates were given subcutaneously, intravenously, and intraperitoneally. Significant increases were shown in the respiratory quotients and marked drops were observed in the blood sugar level (frequently to points below normal) and marked changes were demonstrated in the D:N ratio. These changes were demonstrated both for Alkaline and for acid perfusat'es, but were greater and more constant with acid perfusates given neutral.
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