The Concurrent Expression of Griffonia simplicifolia -IB 4 Binding and Tumor Necrosis Factor-α Differs between Alveolar and Peritoneal Macrophages

Abstract

Abstract

As a corollary to their anatomic location, alveolar macrophages (AM) have a lower threshold for generating some physiologic functions than peritoneal macrophages (PM). In this study, we examined both of these populations for their ability to bind the lectin Griffonia simplicifolia-IB₄ (GSIB₄) and to produce tumor necrosis factor (TNF)-α. The results showed that these two responses were concurrently expressed in activated macrophages, although they differed in magnitude when AM and PM were compared. Following in vitro incubation, AM from lipopolysaccharide-treated rats demonstrated a higher percentage of GSIB₄ positivity and TNF production when compared with their respective PM. Since prostaglandin E₂ can regulate the expression of some macrophage activities, experiments were conducted to determine whether this could also affect the ability of macrophages to bind the GSIB₄ lectin. Neither the administration of indomethacin nor exogenous prostaglandin E₂ altered the expression of this marker. Conversely, these treatments produced significant changes in TNF-α production in both alveolar and peritoneal macrophages. When the concurrent expression of GSIB₄ lectin binding and TNF-α production was analyzed, AM from lipopolysaccharide-treated rats demonstrated both superior GSIB₄ positivity and TNF-α production compared with all other macrophages examined. The results of this work show that AMand PMdiffer in their expression of GSIB₄ binding and TNF-α production. These differential responses may be important in determining the level of activity of macrophages that are participating in an immune response.