Abstract
Abstract
In the intact kidney, renal proximal tubule cells accumulate p-aminohippurate (PAH) via a basolateral, probenecid- and sodium-sensitive transport system. Primary cultures of rabbit proximal tubule cells retain sodium-glucose co-transport in culture, but little is known about PAH transport in this system. Purified proximal tubule cells from a rabbit were grown in culture and assessed for PAH and α-methyl-D-glucoside uptake capacities as well as proximal tubule marker enzyme activities. Control PAH uptake on collagen-coated filters (20 ± 3 pmol/mg protein · min; n = 8) was not significantly different from uptake in the presence of 1 mM probenecid (19 ± 4 pmol/mg protein min; n = 8). Uptake from the basal side of the cell was 3.9 ± 0.7 times greater than that from the apical side. In multi-well plate studies, the uptake was significantly reduced by removing sodium from the medium and stimulated by coating the wells with collagen. Glutarate (10 mM) had no effect on the uptake of PAH. Other differentiated proximal tubule characteristics were retained in culture, including the ability to form domes and to transport glucose by a phlorizin-sensitive system. Phlorizin-sensitive 1 mM α-methyl-D-glucoside uptake was 134 ± 42 pmol/mg protein min (n = 7; P < 0.02). The proximal tubule marker enzymes alkaline phosphatase and γ-glutamyltranspeptidase, increased in activity in the cultures after confluence. It was concluded that whereas some differentiated properties were retained during primary culture of rabbit proximal tubule cells, the PAH transport system was selectively lost or modified from that present in the intact kidney. [P.S.E.B.M. 1992, Vol 199]
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