Effect of Thyroxine and Chicken Growth Hormone on Immune Function in Autoimmune Thyroiditis (Obese) Strain Chicks

Abstract

Abstract

The effect of thyroxine (T₄) and/or recombinant chicken growth hormone (rcGH) supplementation on immune function and on immune cell maturation was examined in Obese strain chickens. Day-old Obese strain chicks received the control treatments or were treated with either T₄ (supplemented in the diet), T₄-rcGH, or rcGH (by daily injection) in a full factorial design. At 4 weeks of age, the proliferative activity of peripheral blood T cells to either mitogenic or allogenic cell (mixed lymphocyte response) challenge was assessed. At the same time, peripheral blood lymphocytes and thymocytes were collected and prepared for flow cytometry analysis. Proliferative responses to both T cell mitogens and allogeneic splenocytes were significantly increased (P < 0.05) by rcGH treatment, while the combined T₄-rcGH treatment resulted in a significant increase in allogeneic and in concanavalin A responsiveness, but not in the response to phytohemagglutinin. All supplemented groups showed a significant decrease in the mean fluorescent intensity for CT-1a⁺ thymocytes, while thymocytes from birds receiving either T₄ or rcGH alone had higher proportions of CD4⁺ and CD8⁺ cells. The monoclonal antibody staining of thymocytes from T₄-rcGH-supplemented animals more closely resembled that of the unsupplemented controls. Among the peripheral blood lymphocytes, there were no changes in the numbers of CD4⁺, CD8⁺, or slg⁺ cells as a result of treatment. The mean fluorescent intensity of slg⁺ cells was significantly decreased, however, as a result of T₄ supplementation when given either alone or in combination with rcGH. Finally, the mean fluorescent intensity ratios of CD4⁺ to CD8⁺ cells was significantly increased as a result of rcGH supplementation. These results strongly support a role for both the thyroid hormones and growth hormone in regulating and/or enhancing immune function, with changes in functional responses paralleled by concomitant changes in the T cell populations as expressed by shifts in T cell surface marker expression.