Abstract
Abstract
Exposure of isolated rat gastric mucosal cells to 10-10 and 10-9 M gastrin (G-17-I) for 2 hr significantly stimulated [3H]leucine incorporation (15 min pulse) into protein by 100 and 212%, respectively, when compared with the basal levels. Doses beyond 10-9 M lowered the maximal stimulatory effect of the hormone. Gastrin (10-9 M) specifically stimulated the synthesis of five proteins in isolated gastric mucosal cells with apparent molecular weights of 105, 76, 71, 63, and 54 kDa. Actinomycin-D (10 μg/ml) completely abolished the gastrin-mediated stimulation of protein synthesis in isolated gastric mucosal cells.
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