Abstract
Abstract
Radioimmunoassay (RIA) methodology for purposes of monitoring immune response of female mice to injection with porcine zonae pellucidae was investigated. Titers of antibodies prepared against zonae pellucidae and, as measured by RIA, were compared to those obtained by the two frequently used zona antibody titration methods of (i) antibody produced precipitation layer formation on the zona surface (PPT titer), and (ii) indirect immunofluorescence (IF titer). RIA 30% binding and endpoint titers produced comparable immune profiles which indicated rapid rise in anti-zona activity occurring during the first 4 weeks following immunization, peak titer achieved between approximately 5 and 8 weeks followed by a very gradual decline in titer. Anti-zona titers remained at significantly high levels at 225 days following injection. RIA endpoint titers were approximately 200 times greater than the IF titers which, in turn, were 32-64 times greater than PPT titers. These data emphasize the greater sensitivity of the RIA over existing methodology for titrating zona antibodies and the feasibility for using RIA procedures for monitoring immune response following injection with zona material.
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