Abstract
Abstract
Mouse brain antisera with anti-CFU-S activity was utilized to evaluate the role of the pluripotent stem cell (CFU-S) in a diffusion chamber (DC) myelopoiesis. Antisera incubation with marrow resulted in a 93 ± 2% reduction in detectable CFU-S in the standard spleen colony assay and a variable reduction in the granulocyte-monocyte progenitor cell (CFU-C). Marrow virtually devoid of detectable CFU-S and containing variable CFU-C relative to control demonstrated equal or greater numbers of CFU-S, CFU-C, and differentiated progeny at varying time intervals after DC implantation. These results may indicate that neither the CFU-S nor the CFU-C is the progenitor cell for DC growth. Alternatively, CFU-S antibody may not kill CFU-S in vitro or in the in vivo DC system but may opsonize the cell prior to eventual reticuloendothelial destruction. The latter concept is supported by reversal of antisera effect on CFU-S recovery after in vitro exposure of antisera-treated cells to pronase.
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