Abstract
Abstract
Hepatocytes isolated from rats deprived of water for 48 hr (dehydrated) were compared with hepatocytes from control rats with respect to four effects of vasopressin: the inhibition of glucose-promoted glycogen synthesis; the stimulation of glycogenolysis in the absence of exogenous glucose; glucose production; and pyruvate decarboxylation. Vasopressin (ADH) prevented net glycogen synthesis in hepatocytes from both control and dehydrated rats. The inhibitory effect of ADH on hepatocyte glycogen synthesis disappeared within 45 min of washing the cells with hormone-free medium. Vasopressin stimulated glycogenolysis by 60% and glucose output by 80% in hepatocytes from either control or dehydrated rats. In 24-hr-fasted rats, prior dehydration had no effect on either basal or ADH-stimulated glucose production or pyruvate decarboxylation. Thus no evidence was found that water deprivation and the associated prolonged exposure to elevated plasma ADH led to adaptive changes in carbohydrate metabolism or in the ability of hepatocytes to respond to ADH.
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