Effects of Organ Preservation on the Transport of RNA from the Nucleus

Abstract

Summary

The transport of kidney nuclear RNA from the nucleus to the cytoplasm in an in vitro system was determined after ischemic injury to the tissue. The transport of the [³H]RNA was decreased to approximately half that of the control level by 1 hr of warm ischemia or 4 hr of cold ischemia. The transport control is energy dependent. After prolonged cold storage, 24 to 72 hr, the release of [³H]RNA from the nucleus to the cytoplasm increases. This increased release of RNA that occurs with ischemia in the cold is less energy dependent and presumably not representative of in vivo mechanisms.