Abstract
Abstract
Envelope and core proteins of Venezuelan encephalitis virus were detected by indirect fluorescent antibody staining in cultured primary hamster embryonic cells infected under single-cycle conditions. Antibody to purified core protein stained the entire cytoplasm at 7 hr postinfection, but never the outer surface of the plasma membrane. By 24 hr postinfection, when cytopathic effects were extensive, core protein was present as cytoplasmic granules. Antibody to purified envelope proteins stained a single bright perinuclear area in less brightly fluorescing cytoplasm at 7 hr postinfection, and plasma membranes at 8 hr postinfection. The perinuclear location of Venezuelan encephalitis virus envelope glycoproteins was interpreted as involving the Golgi apparatus which is a known location of intracellular glycoprotein synthesis. Thus, the Golgi apparatus is apparently a source of the smooth membranes with which α-virus envelope proteins have been associated after cell fractionation.
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