Ammoniuretic Activity of Dog Plasma When Tested on the Urinary Bladder of Bufo marinus

Abstract

Abstract

Hemibladders from the toad Bufo marinus were used in all experiments. A protein free preparation of plasma was made from dogs in metabolic acidosis, respiratory acidosis, and metabolic alkalosis. These plasma preparations were tested for ammoniuretic activity by placing them on the serosal side of the urinary hemibladders of Bufo marinus mounted in vitro. Both acidotic plasma preparations caused increased ammonia excretion into the mucosal media as compared to control hemibladders. Metabolic alkalotic plasma preparations did not produce significant ammoniuretic activity. Using paired hemibladders, the effect of metabolic acidotic plasma preparations showed increased ammoniuretic activity when compared to metabolic alkalotic plasma preparations (P < 0.05).