Attempt to Demonstrate Virus Interference in Cell Cultures Persistently Infected with the Viruses of Kuru and Creutzfeldt-Jakob Disease 1

Abstract

Summary

Cell cultures persistently infected with kuru and CJD viruses were challenged with 18 different viruses in an effort to demonstrate in vitro interference. Cell cultures derived from the brains of a normal chimpanzee and a chimpanzee affected with kuru and CJD were challenged with the following viruses: herpes simplex, vaccinia, measles, SV-5, parainfluenza (types 1 and 3), reovirus (types 1, 2, and 3), vesicular stomatitis, Tacaribe, Sindbis, Bunyamwera, Japanese B encephalitis, St. Louis encephalitis, Langat, Powassan, and chimpanzee adenovirus (Pan 5). There were no significant differences in titers of any of these viruses, respectively, in the kuru, CJD, or normal chimpanzee cell lines. In addition, each of the 10 challenge viruses showed no significant differences in titers, respectively, in Vero or PK-15 cells preinoculated with high-titered brain suspensions from man or chimpanzees with kuru or CJD or normal chimpanzee brain.