Abstract
Summary
Human sera or plasma containing acid phosphatases were mixed with rabbit antiserum specific for prostatic acid phosphatase (PAP) and the resulting primary immune complexes were precipitated by ammonium sulfate. The amount of specifically precipitated PAP was estimated directly by its enzyme activity. Using this assay, as little as 10 ng/ml of the PAP could be determined. Excellent correlation of this assay method with other immunoassays of PAP was demonstrated.
This work was supported in part by PHS Research Grant CA 18748 and CA 16426 from the National Cancer Institute.
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