Abstract
Summary
When the marrow and spleen cells of plethoric mice are cultured with 1.0 unit/ml of limulus-lysate-absorbed Ep, the predominant erythroid progenitor cell assayed is the Day 3 BFU-E. Erythropoiesis by these cells can be studied by enumeration of bursts at 3 days or by 24-hr 59Fe incorporation into heme. Endotoxin markedly increased the number of Day 3 BFU-E in the spleens of these mice and decreased the number in the marrow, but it had little effect on erythropoietic maturation. In contrast, Ep produced differentiation of the erythroid progenitor cells resulting in increased splenic CFU-E and a decline in the more primitive erythroid precursor cells.
We wish to thank Ms. A. Brockman for excellent technical assistance. Mark J. Koury is the recipient of a research fellowship supported by an NIH Postdoctoral Training Program in Hematology, Grant T32 AM07186. This work was supported in part by Grant AM15555 from the National Institute of Arthritis, Metabolism and Digestive Diseases, by the Medical Research Service of the Veterans Administration and by the Brownlee O. Curry Leukemia Research Fund.
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