Abstract
Summary
The use of either D-valine-sub-stituted medium or BUdR treatment have been shown to be effective in reducing fibro-blastoid cell proliferation in pancreatic monolayer cell cultures established from neonatal rats. Maintenance of these cultures in D-valine medium appeared to completely curtail further culture growth, but this was apparently accompanied by a loss of both A and B cells as evidenced by a fall in both glucagon and insulin release. A single 24-hr treatment of cultures with BUdR was just as effective in curtailing fibroblastoid cell growth, but within a few days following such treatment, fibroblast proliferation resumed, although the number of fibroblasts remained reduced compared to control cultures. On the other hand, with BUdR treatment continued loss of A and B cells apparently did not occur.
I am grateful for the excellent technical assistance of Ms. Jeanette Teague in the performance of these studies, and the expert preparation of this manuscript by Ms. Sharon Kemp.
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