In Vitro Inhibition of the Rate of Erythrocyte Sickling by RMI 11,071A and Its Possible Mechanism

Abstract

Summary

RMI 11,071 A, one of a series of lactamimides, was found to inhibit the sick-ling rate in an in vitro system. This inhibition was not total or permanent; the sickling rate increased after 30 min at low O₂ tension and the number of sickled cells approached that of the controls by 60 min. There was no effect on pH, pCO₂, or the oxygen dissociation curve. ⁴⁵Ca fluxes were affected. Though influx of ⁴⁵Ca increased on deoxygenation, the compound caused a decrease in ⁴⁵Ca content on reoxygenation. This decrease was absent in the control group.