Abstract
Summary
This report describes the in vitro biological properties of an osteosarcoma virus, FBR, which was originally isolated from a 90Sr-induced osteosarcoma in an X/Gf mouse. The virus complex consists of a transforming component, MuSV, and a nontrans-forming component, MuAV. Foci of cells transformed by FBR-MuSV(MuAV) are late appearing and consistently yield single-hit focus titration patterns. The isolate is infectious for only murine cells and shows distinct b-tropism. A clone of transformed cells yielding high-titered sarcoma-rich virus has been propagated for the large-scale production of this virus. Tissue-culture-derived FBR-MuSV(MuAV) possesses osteosarcomagenic properties comparable to those of the parent virus.
This research was jointly supported by the U.S. Department of Energy and by Contract Y01 CP 7-0504 from the Divison of Cancer Cause and Prevention, National Cancer Institute. We are indebted to Dr. T. E. O'Connor for his valuable discussions. We thank Marian F. Williams and our summer and spring undergraduate students, Nancy E. Fay and Marcia L. Zucker, respectively, for their excellent assistance, and Isabel Greco for graphic and photographic illustrations.
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