Abstract
Summary
The role of spleen lymphocytes in acute and latent guinea pig herpes-like virus (GPHLV) infection and the in vitro susceptibility of the lymphocytes to GPHLV were explored. Macrophage-, B-cell-, and T-cell-enriched populations obtained from infected guinea pigs were examined for infectious GPHLV. During acute infection, virus was first detected in the macrophage and B-cell fractions, whereas, infectious virus was only evident in the T-cell fraction 5 days or more after inoculation. During latent infection, infectivity titers in the B fractions were consistently higher than in the T fractions. In both the B and the T lymphocytes derived from latently infected guinea pigs, virus was expressed only after in vitro cultivation or cocultivation with susceptible cells. Lymphocytes infected in vitro did not support GPHLV replication, although latent infection of lymphocytes with GPHLV was readily accomplished in vivo.
We thank Christine Cote for excellent technical assistance. This research was partially supported by a Research Training Grant 5 T32 AI 07018 from the Institute of Allergy and Infectious Diseases, National Institutes of Health, and by the Medical Research Service of the Veterans Administration.
Get full access to this article
View all access options for this article.