Abstract
Summary
Propagating stationary suspension cultures of a cell line (Ta-4) derived from the IR162 IgE secreting rat myeloma produced high concentrations of IgE, formed colonies in agarose solidified medium, and when injected into rats induced tumors similar to the original myeloma. The in vitro produced IgE was capable of binding to rat skin mast cells. A system was developed to assay the effects of drugs on IgE synthesis and results with 5-bromo-2′-deoxyuridine indicate that IgE synthesis can continue at high levels in the absence of cell replication.
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