Abstract
Summary
Three antigens (Ln-l-Ln-3) in extracts of normal rat liver were detected by immunodiffusion analysis using rabbit antibodies to liver nucleoli. In analyses of a series of tissues, antigens Ln-1 and Ln-3 were found only in normal and regenerating liver. Antigen Ln-2 was found in several tissues studied but not in kidney or rat serum; in kidney, an antigen was found that formed an overlapping band with antigen Ln-1 but did not exhibit identity with antigen Ln-1. In liver, approximately 99% of the antigen Ln-1 was in the cytosol and 1% was in the nuclei. In the extracts of nuclei and nucleoli, approximately 6-8% of this antigen was in the chromatin fraction from which it was extractable with 0.6 M NaCl. The antigens were purified on affinity columns which contained covalently bound liver antinucleolar antibodies. Interestingly, the bound antigen contained GC-rich RNA of approximately 200,000 MW.
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