Abstract
Summary
The effects of hyperthermia and ascorbic acid upon the metabolism of [U-14C] glucose, [1-14C] pyruvate, and [1,5-14C] citrate by EATC were examined. All experiments were conducted at 37.5° and 42.5° in the presence or absence of ascorbic acid. Hyperthermia treatment for 2 and 3 hr significantly decreased cell viability as determined by trypan blue exclusion. Ascorbic acid did not affect cell viability. Hyperthermia did not significantly alter EATC metabolism of glucose, pyruvate, or citrate. However, ascorbic acid (1 mM) significantly increased the metabolism of all three compounds by EATC. Neither hyperthermia or ascorbic acid had any significant effect upon the metabolism of [U-14C] glucose by kidney slices or minced spleen.
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