Abstract
Pancreatic and malt amylase and the amylase of Aspergillus oryzæ (prepared from taka-diastase) have been selected as representative of the starch-splitting enzymes of the higher animals, higher plants and fungi respectively. Laboratory methods for the purification of each of these amylases have been described in previous papers. The present experiments were performed with enzyme preparations which had been purified in accordance with these methods. The experiments establish for each of the three amylases the limits of hydrogen ion concentration within which any enzymic activity is shown, and the form of the curve representing the activities at all concentrations of hydrogen ion between these limits. The investigation was carried out with the aid of a grant from the Carnegie Institution of Washington.
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