Inhibition of [ 3 H]TdR and [ 3 H]CdR Incorporation in an Established Human Lymphoma Cell Line by Trypsin Treatment 1

Abstract

Summary

[³H]TdR and [³H]CdR incorporation into intact human lymphoma cells was reversibly inhibited by brief treatment with proteolytic agents (trypsin and Viokase). Neither enzyme affected the incorporation of the tritiated nucleosides by HeLa or human melanoma cells. These results are different from those described for rodent B-lymphoid cells and indicate that protease treatment does not invariably lead to increased DNA synthesis.