Abstract
Summary
Specific, high titer anti-GnRH antisera were obtained following immunization of rabbits with a conjugate of thyroglobulin and D-[Lys6]-GnRH. D-[Lys6]-GnRH was chosen to take advantage of an ∊-amino group in the center of the peptide chain and three carbons away from the chain as a unique point of conjugation. The RIA developed with this antisera was validated for the measurement of GnRH in plasma and hypothalamic supernatants. Studies of GnRH metabolic clearance indicated that multicompartmental clearance by different organs was a probable explanation for the rapid disappearance of GnRH from the peripheral circulation.
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