Isolation of Highly Enriched Preparations of Two Types of Mucosal Cells of the Turtles Urinary Bladder

Abstract

Summary

Enriched preparations of the two major cell types of the mucosal epithelium of the turtle's urinary bladder can be obtained by density gradient centrifugation. Carbonic anhydrase activity is much greater in the mitochondria-rich than in the granular cell, suggesting the former cell type is the site of acetazolamide-sensitive transport. This technique should be useful in further defining the biochemical and transport properties of epithelial tissues composed of heterogeneous mucosal cells.