Abstract
Summary
The growth-promoting activities of a number of lipid-related chemical compounds were studied in C. briggsae. It was found that several such compounds, Tween 80 (20 mg/ml), Tween 85 (10 mg/ml), sodium oleate (1.0 mg/ml), sodium stearate (1.0 mg/ml), ethanol (4.0 mg/ml), n-propanol (4.0 mg/ml), and potassium acetate (5.0 mg/ml), greatly stimulated population growth in C. briggsae and were much more potent than a casein hydrolysate (casamino acids). These findings have led to the recognition of a lipid-related factor for C. briggsae and to the successful development of a completely chemically defined medium for the cultivation of C. briggsae without the presence of a protein-aceous factor.
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