Abstract
Summary
Unfractionated marrow cell suspensions provide adequate numbers of mouse neutrophils for studies of spontaneous and chemotactic migration under agarose. Mature neutrophils were the only cells to leave the wells in the first 4 hr. For chemotactic studies, quantitation involved microscope counts of cells in selected samples of the migration areas, and the chemotactic responses were linearly related to the dilution of activated serum. Mouse, rat, guinea pig or rabbit activated sera but not human sera were chemotactic for mouse granulocytes. Spontaneous migration was determined by total counts of neutrophils leaving the wells.
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