Abstract
Summary
Three transplantable sublines of WEHI-3B myelomonocytic leukemia in BALB/c mice were developed by splenic (SP), intraperitoneal (IP) and subcutaneous (SQ) passage. All three sublines then were compared for stability on passage, lethality for mice, and major hematologic and histopathologic effects. The time in days until death was a linear function of the log10 dose of viable cells injected for each cell line. Evidence was provided that a single cell of the IP line was lethal to mice and that death from all three cell lines approximated single cell kill. All three sublines were stable after adaptation by serial passage. Detailed hematologic and histopathologic studies were done on mice that were inoculated iv and ip with the SP and IP sublines. Changes typical of myelomonocytic leukemia were observed except that bone marrow invasion was minimal.
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