Effect of the Prolonged Immunization with Tumor-Unrelated Antigen on the Tumor Growth 1

Abstract

Summary

The effect of prolonged immunization of mice with bovine serum albumin (BSA) or ovalbumin (OA) on the growth of the transplanted, methylcholanthrene-in-duced fibrosarcoma was studied. When immune complexes appeared in the kidneys and in the circulation, mice were injected subcutaneously with various numbers of syngeneic tumor cells.

Tumor developed within 17 days in all mice immunized with BSA and injected with 10⁴, 10³ and 10² tumor cells and control mice injected with 10⁴ and 10³, whereas only 3 of 10 control mice injected with 10² tumor cells developed tumors at that time. All mice immunized with OA and injected with 10⁵, 10⁴ and 10³ tumor cells and control mice injected with 10⁵ and 10⁴ developed tumor within 12 days after injection of the tumor cells, whereas in the control group injected with 10³ tumor cells, only 6 of 12 mice did so. Weight of the tumors from the mice immunized with BSA or with OA was significantly greater than that of tumors from control mice.