Abstract
Summary
Lymphoblastoid cell lines were derived from peripheral blood leukocytes of woolly monkeys by exposure to the B95-8 strain of EB virus. Primary cells were relatively resistant to transformation and the resulting lines produced little virus. Almost half the lymphocytes in fresh blood had a receptor for IgFc and 60-90% of continuously cultured cells expressed receptors for both rabbit and human IgFc as detected with the “EA” rosette test. The IgFc receptor on woolly monkey cell lines was qualitatively different from that induced by herpes simplex virus which could be detected with rabbit but not human Ig. Monkeys inoculated with cell-free virus and autologous transformed cells remained well and showed no evidence of infection. There was thus no evidence that woolly monkey cells transformed in vitro by EBV were oncogenic when replaced in the autologous host.
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