Heterogeneity of KCl-solubilized Antigens of Chemically Induced Sarcomas 1

Abstract

Summary

Solubilized antigens of murine methylcholanthrene-induced fibrosarcomas prepared by treatment of tumor tissue with hypertonic KCl were separated into fractions depending upon exclusion by molecular weight by selected ultra-filters. These studies showed that material of both high molecular weight (>300,000) and low molecular weight (<30,000) inhibited ³H-thymidine incorporation by lymphoid cells taken from tumorbearing animals. When the inhibitor were removed, the material remaining stimulated proliferation in such lymphoid cells over a wide range of molecular weights (<30,000 daltons to > 100,000 daltons).