Abstract
Summary
The relative effectiveness of sonication and 3 M KCl extraction for solubilizing antigens from line Ib malignant lymphocytes were compared. Electron microscopic studies provided evidence that 3 M KCl extracts contained fewer membrane fragments and more closely approximated a rigorous definition of “soluble.” Sephadex G-150 gel filtration data indicated that the solubilized preprations contained three main peaks, viz, the void volume, a molecular species <; 69,000 and a molecular species < 14,000. Fractions eluted from Sephadex columns elicited immune polioenceph-alomyelitis in indicator mice. Solubilized preparations immunized mice to the disease. Preparations containing membrane fragments immunized C58 mice to trans-plantable line Ib leukemia
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