Abstract
Summary
Feline embryo fibroblast cells were found to be very sensitive to transformation by the primate RNA tumor viruses, simian sarcoma virus (SiSV-1) and HL23V, a virus isolated from the leukemic myelo-blasts of an AML patient. In vitro transformation of feline embryo fibroblast cell cultures with these viruses was 2 to 100 x more efficient than when performed on the normal rat kidney (NRK) or rat embryo fibroblast cell lines commonly used for SiSV-1 transformation studies. Several cell lines of marmoset and human origin were also observed to be less sensitive to transformation by these viruses. Furthermore focus development on feline embryonic fibroblast cultures required only 3-4 days compared to 7-10 days needed using rat or primate cultures.
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