Abstract
Summary
Herpes simplex virus infection was studied in in vitro cultured mouse neuroblastoma (C 1300) cells displaying many properties characteristic of differentiated neurons. High multiplicity of infection caused a cytocidal type of infection. At a low multiplicity of infection, cultures persistently or only occasionally releasing virus into the culture fluid were established. These cultures have been subcultured more than 40 times. C 1300 Cells produced hundred- to thousand-fold less HSV per cell than a number of other cell cultures of murine, simian, or human origin. About 1% of the C 1300 cells in a culture persistently releasing virus was found to carry HSV-induced antigens. Since HSV antiserum added to the persistently infected cultures efficiently cleared cultures of infective virus, cell-to-cell spread of virus seemed of minor importance.
This work was supported by the Swedish Medical Research Council (Project No. 4514).
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