Abstract
Summary
The effect of DBcAMP on reaggregates of dispersed spinal and sympathetic ganglia, kidney, and liver cells is examined in order to define the nature of the growth acceleration that occurs when nerve is cultured with parenchymal tissue. Single cell suspensions prepared from chick embryo tissue are permitted to reaggregate on a rotary shaker. Measurement of the incorporation of labeled precursors into DNA and protein is used to monitor the effect of DBcAMP on the cultured reaggregates. The stimulatory effect of nerve on the growth and proliferation of kidney and liver reaggregates is mimicked by low concentrations of DBcAMP, suggesting a possible role for cyclic nucleotides in the growth processes of embryonic parenchymal organs.
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