Effect of Petroselinic and Stearic Acids on the Alkyl Diacyl Glycerides of Novikoff Hepatoma Cells 1

Abstract

Summary

Differences in the metabolism of petroselinic (n 12-18:1) and stearic (18:0) acids were compared in Novikoff hepatoma cells cultivated in vitro. Addition of 100 μg of 18:0/ml to the growth medium of the cells increased their size and the concentrations of polar, neutral, total lipid, and protein, as well as the dry weight. Inclusion of the same amount of n12-18:1 in the culture medium produced similar changes but of a smaller magnitude than those of 18:0. Cells grown in the presence of 18:0 contained an amount of alkyl diacyl glycerides (ADG) similar to that found in cells grown in unsupplemented medium, whereas the cells grown in culture with n 12-18:1 contained only about 33% of this amount of ADG.

There was no change in the amount of total lipid with time of incubation when the cells were grown in unsupplemented medium, whereas when the cells were grown in the presence of n12-18:1 or 18:0, 30 and 25%, respectively, of the total fatty acids were utilized in 48 hr.