Abstract
Summary
The role of an altered ribonu-cleotide diphosphate reductase activity in the resistance of equine herpesvirus type 1 to hydroxyurea was demonstrated. A 28% increase in total CDP reductase was observed by 6 hr PI, and the enzyme activity of EHV-l-infected cells differed from that of mock-infected cells in several properties. Infected cell activity (i) did not require ATP for in vitro activity, (ii) exhibited a lower apparent K m, and (iii) demonstrated a decreased sensitivity to HU added directly to the reaction mixtures. The altered CDP reductase activity was first detected at 3-4 hr PI, the time of initiation of viral DNA synthesis.
Support for this investigation was obtained by U.S. Public Health Service Research Grants AI 02032 and AI 08421, by Training Grant AI-69, and by No. General Research Support Grant No. RR-05386, Division of Research Resources.
Get full access to this article
View all access options for this article.