Abstract
Summary
Lymphocytes from seven patients suffering from localized skin melanoma were mixed with melanoma cells derived from an established in vitro cell line, shown to be tumorigenic in nude mice but without tendency to invasive growth or formation of distant metastases. These lymphocyte-tumor cell suspensions were inoculated sc into nude mice. Comparable inoculations were performed with lymphocytes derived from seven healthy control donors. The L/T ratio was 10/1. Three parameters were measured: tumor incidence, growth rate, and latency period. It appeared that patient lymphocytes decreased the number of tumor takes and increased the latency period. The growth rate was not decreased by the patient lymphocytes. These findings support the general assumption that lymphocytes derived from melanoma patients are sensitized against a group-specific antigen.
The Fibiger Laboratory is sponsored by the Danish Cancer Society.
The technical assistance of Vibeke Ahrenkiel and Marianne Barfoed is gratefully acknowledged.
This work was supported by the Danish Medical Research Council Grants 512-5274 and 512-3547.
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