Abstract
Summary
When added to the media of cultures of retrobulbar fibroblasts, GlcN, a precursor of HA, increased the synthesis of, and was readily incorporated into, HA. The formation of GlcN by the fibroblasts appears to be rate limiting in the biosynthetic pathway of HA.
Media enriched with GlcN potentiated the stimulations of HA production induced by lymphocytes and DbcAMP. The poten-tiation related to an expanded intracellular quantity of GlcN. Increasing the GlcN available to the fibroblasts resulted in a maximum HA synthesis which could be exceeded by the simultaneous presence of DbcAMP. There may be a cAMP-dependent step in HA synthesis subsequent to the formation of glucosamine.
Dr. C. W. Castor provided valuable advice in the preparation of this manuscript. Expert technical assistance provided by Piyush Kothary, Deborah Weiker, and Howard Kirchick was greatly appreciated. The author is also pleased to acknowledge the expert editorial assistance of Cornelia Osborn.
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