Abstract
Summary
A new derivative of arsonic acid, arsanil isothiocyanate was used to couple arsonic acid via an is othiocyanate group to amino groups. Immunization of guinea pigs with this determinant attached to e-amino carproic acid in complete Freund's adjuvant led to the production of delayed hypersensitivity toward this haptenic determinant, as indicated by positive skin tests to compounds to which the determinant was coupled to egg albumin and bovine serum albumin. In addition, lymphocytes from these immunized animals responded to these antigens in vitro by undergoing blast transformation as measured by incorporation of tritiated thymidine.
The dominance of the arsonic acid group in the determinant was seen in the form of positive skin tests as well as in in vitro stimulation with the various conjugates in animals immunized with either Ars-NCS-EACA or ABA-tyr.
The response by the homologous compounds were, however, generally better than those of the heterologous compounds. These results suggest an important contribution to the reactivity of the immunodomi-nant arsonic acid group by the neighboring site of attachment.
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