Abstract
Summary
It is shown that appropriate xenogeneic rabbit and horse antiserum, as well as allogeneic rat serum, can serve as sources from which 125I-labeled antibody to a strong histocompatibility antigen expressed on Fischer-344 strain rat cells can be prepared. After iv administration all three types of antibody would localize preferentially in F-344 skin grafts made 4 days earlier on Buffalo strain rats. After 24 hr, 125I localization ranged from 10 to 20% of the injected dose on F-344 skin grafts averaging 0.3 g weight on 150-190-g Buffalo rats. The horse serum used as an antibody source was a commercially available anti-rat-lymphocyte serum. Prior ip administration of two or three doses of 0.5 ml of this serum largely blocked uptake by F-344 skin grafts of both the 125I-labeled alloantibody and the 125I-labeled xenogeneic histocompatibility antibody prepared from this horse antiserum.
This work was supported by USPHS Research Grant CA-16749 from the National Cancer Institute and under contract with the U.S. Energy Research and Development Administration with the University of Rochester and has been assigned Report No. UR-3492-913
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