Abstract
Summary
A method of aging cells is described which allows for an increase in sensitivity of the assay of interferon. After reaching confluency the cell cultures are aged at 30° for 5 days, the growth medium is replenished, and the cells are incubated for an additional 24 hr at 37° before applying interferon. Using cell cultures so treated (dip-loid cells HFS-1 or HR-203), and mouse L-929 cells), the sensitivity of the interferon assay increase from 16- to 30-fold for human and up to 10-fold for mouse standard interferon.
We are grateful to J. A. Green, C. E. Buckler, and S. Baron for helpful advice.
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