Appearance of Inorganic Pyrophosphatase during Growth of Streptococcus mutans Cells 1

Abstract

Summary

It is important for the purpose of obtaining PP₁ase activity to promptly harvest 5. mutans KlR cells at the height of the log phase of growth or an OD of 0.5 at 660 nm. The apparatus shown in Fig. 1 enables one to grow streptococcal cells and to determine specifically during the growth cycle when to harvest the cells. Of the five cell disruption techniques tried, ultrasonic treatment is obviously the technique of choice for disruption of S. mutatis KlR cells and solubilization of PP_iase for maximum yield.